Ain Shams University, Egypt
Title: Association of long noncoding RNA and c-JUN expression in hepatocellular carcinoma patients
Ahmed E M Azazy is a fourth year Medical Cadet at Armed Forces College of Medicine (AFCM), active member at International Genetic Engineering Machine (IGEM). He has published three papers in international journals.
Background: Long noncoding RNAs (lncRNAs) have emerged as key elements in modulating gene expression in different biological contexts. Accumulating evidence indicates that lncRNAs are strongly implicated in hepatocellular carcinoma (HCC) development and progression.
Objectives: Long non coding RNA-urothelial cancer associated 1 (lncRNA-UCA1) and cellular jun proto-oncogene (c-JUN) have been scheduled in this study to discuss their possible association in HCC patients based on bioinformatics tools and clinical validation.
Patient & Methods: We used quantitative real-time PCR (QPCR) to evaluate the expression of lncRNA-UCA1 and C-JUN in serum of 70 patients with HCC, 32 patients chronic hepatitis C (CHC) and 38 healthy subjects and their correlation with different clinicopathological factors. The prognostic significance of lncRNA-UCA1 and c-JUN were tested by Kaplan-Meier survival analysis.
Results: The expression of lncRNA-UCA1 and C-JUN was positive in 91.4% HCC patients with strong discriminating power between HCC and healthy subjects and CHC patients as well. The median follow up period was 29 months. The survival analysis showed that both lncRNA-UCA1 and C-JUN were independent prognostic factors. Of note, we identified C-JUN expression changes consistent with the lncRNA-UCA1 target regulation.
Conclusion: Our study sheds light on the possible role of lncRNA-UCA1 and C-JUN mRNA as promising diagnostic and prognostic markers as well as potential therapeutic targets in HCC.